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Hi,
I am new to miRNA seq analysis. Now I want to use fastx_clipper to trim the adapters from my miRNA seq data. For fastx_clipper, there is an argument:
[-M N] = require minimum adapter alignment length of N.
At beginning, the parameters I used is :
fastx_clipper -v -Q 33 -l 18 -v -a TGGAATTCTCGGGTGCCAAGG -c
But when I add the argument -M 8, the results would not be the same. Actually another 2000 reads were clipped.
Can anyone tell me the default -M N value in this tool? And how does it work?
Thanks.
I am new to miRNA seq analysis. Now I want to use fastx_clipper to trim the adapters from my miRNA seq data. For fastx_clipper, there is an argument:
[-M N] = require minimum adapter alignment length of N.
At beginning, the parameters I used is :
fastx_clipper -v -Q 33 -l 18 -v -a TGGAATTCTCGGGTGCCAAGG -c
But when I add the argument -M 8, the results would not be the same. Actually another 2000 reads were clipped.
Can anyone tell me the default -M N value in this tool? And how does it work?
Thanks.
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