Dear all, If we run Bowtie2 at default parameters then who do we know that how many parameters it included actually. through which we can correlate our mapping results. one more thing if we got 20% aligned exactly one time and suppose 16% align more than one times, If I would quantify this, each reads of 20% data UNIQUELY COMPLETELY [uniquely means each reads would be mapped exactly one coordinate of particular chromosome and completely means if read length is 100nts then total 100 nts would be align in continues manner (?)] [is it explainable further??? ]
if we talk about 16% of reads which aligned more than one position, there may be several possibilities: 1. few reads may be overloaded with 20% of reads which are mapped exactly ONE time. 2nd possibility, fraction of a read map different different location with unknown insert size. [any other possibility]
how could I get depth of reads which fall on a particular location of chromosome? what is the maximum occurrence reads adhering at same position at particular chromosomal location? in next step, apart from SAM tool is any other way for downstream analysis ???
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
Latest Articles
Collapse
-
by seqadmin
Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
Long-read sequencing has seen remarkable advancements,...-
Channel: Articles
12-02-2024, 01:49 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 12-02-2024, 09:29 AM
|
0 responses
158 views
0 likes
|
Last Post
by seqadmin
12-02-2024, 09:29 AM
|
||
Started by seqadmin, 12-02-2024, 09:06 AM
|
0 responses
56 views
0 likes
|
Last Post
by seqadmin
12-02-2024, 09:06 AM
|
||
Started by seqadmin, 12-02-2024, 08:03 AM
|
0 responses
48 views
0 likes
|
Last Post
by seqadmin
12-02-2024, 08:03 AM
|
||
Started by seqadmin, 11-22-2024, 07:36 AM
|
0 responses
76 views
0 likes
|
Last Post
by seqadmin
11-22-2024, 07:36 AM
|