Hi, all, since I am some new to RNASeq analysis, I have a lot of questions... Thanks for your help in advance.

My question here is, if the total number of reads for my biological duplicates vary, will that have an effect on the results, for example differential gene expression?

I have several biological duplicates for each group, the total number of reads vary between 80~150M. I will normalize them by RPKM, does this cancel the effect of the difference in reads? If not, what is the way to minimize the effect of these variances in duplicates?

My question here is, if the total number of reads for my biological duplicates vary, will that have an effect on the results, for example differential gene expression?

I have several biological duplicates for each group, the total number of reads vary between 80~150M. I will normalize them by RPKM, does this cancel the effect of the difference in reads? If not, what is the way to minimize the effect of these variances in duplicates?

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