Hello there!
I've just started analysing bacterial metatranscriptome Illumina data. I'm a bit confused on the pipeline to follow. That's what I did:
-I used FastQC to have a general overview of my sequences quality, then I removed the adaptors using the FastX toolkit;
-I assembled the data using Velvet and then Oases, obtaining a file with transcripts.
Now I'm not sure about how going on... I thought to use BLAST for annotation and then mapping the files from each sample individually to the assembled transcriptome using BWA... But then I don't know how proceed... I'd like to have something like this:
GeneID SampleID1 SampleID2 .......
gene1 %abundance %abund
gene2 %abund %abund
....
Then use this table to build some of those awesome plots like heatmaps...
Is there a software that produce an output like this?? Then, does anyone have comments/suggestions on the workflow I used?
Thanks a lot
Francesca
I've just started analysing bacterial metatranscriptome Illumina data. I'm a bit confused on the pipeline to follow. That's what I did:
-I used FastQC to have a general overview of my sequences quality, then I removed the adaptors using the FastX toolkit;
-I assembled the data using Velvet and then Oases, obtaining a file with transcripts.
Now I'm not sure about how going on... I thought to use BLAST for annotation and then mapping the files from each sample individually to the assembled transcriptome using BWA... But then I don't know how proceed... I'd like to have something like this:
GeneID SampleID1 SampleID2 .......
gene1 %abundance %abund
gene2 %abund %abund
....
Then use this table to build some of those awesome plots like heatmaps...
Is there a software that produce an output like this?? Then, does anyone have comments/suggestions on the workflow I used?
Thanks a lot
Francesca
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