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  • arkilis
    replied
    Originally posted by Kennels View Post
    No, the adapters are not the primers.
    5' adapters are what you ligate to your sample sequence (dna, cdna, srna etc) at the 5' end, and 3' adapter at the 3' end. The primers would then anneal to your adapters during PCR amplification.
    thx~~ it is much clear to me now
    Last edited by arkilis; 09-26-2013, 09:24 PM.

    Leave a comment:


  • Kennels
    replied
    No, the adapters are not the primers.
    5' adapters are what you ligate to your sample sequence (dna, cdna, srna etc) at the 5' end, and 3' adapter at the 3' end. The primers would then anneal to your adapters during PCR amplification.

    Leave a comment:


  • NEWBIE question: does the 5' adapter mean forward primer?

    As in the title.

    3' adapter == reverse primer
    5' adapter == foward primer

    Is that right? Thanks!

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