Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Help with script that seperates reads from fastq file

    hi,
    I have fastq files of reads and i would like to finish this script in order to select the "good" reads from the "bad" reads and after that to save my results in new fastq files(good.fastq bad.fastq) .
    Thanks for the help

    gc()
    rm(list=ls())
    library(ShortRead)
    showMethods(readFastq)
    showMethods(writeFastq)
    library(Biostrings)

    #read bait sequences
    baitseq <- readLines("Downloads/bait_seq.txt")
    baitseq

    #choose case
    num <- 1
    exptPath <- ("Downloads/INDEX1_reads.fastq")
    exptPath

    #read data
    fq <- readFastq(dirPath=exptPath)
    sread(fq)

    #count ocurrences of bait
    pos <- grepl(baitseq[num],sread(fq))
    pos[1:25]
    # number of reads
    length(pos)
    # number of hits
    sum(pos)

    # fuzzy match - 2 substitutions
    fpos <- agrep(baitseq[num],sread(fq), max = list(sub = 2, ins=0, del=0))
    fpos[1:25]
    # number of fuzzy hits
    length(fpos)

    # save "good" reads from from fuzzy match in "*.good.fq" file and "bad" reads in *".bad.fq" file


    ?????

Latest Articles

Collapse

  • seqadmin
    Advanced Methods for the Detection of Infectious Disease
    by seqadmin




    The recent pandemic caused worldwide health, economic, and social disruptions with its reverberations still felt today. A key takeaway from this event is the need for accurate and accessible tools for detecting and tracking infectious diseases. Timely identification is essential for early intervention, managing outbreaks, and preventing their spread. This article reviews several valuable tools employed in the detection and surveillance of infectious diseases.
    ...
    11-27-2023, 01:15 PM
  • seqadmin
    Strategies for Investigating the Microbiome
    by seqadmin




    Microbiome research has led to the discovery of important connections to human and environmental health. Sequencing has become a core investigational tool in microbiome research, a subject that we covered during a recent webinar. Our expert speakers shared a number of advancements including improved experimental workflows, research involving transmission dynamics, and invaluable analysis resources. This article recaps their informative presentations, offering insights...
    11-09-2023, 07:02 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 09:55 AM
0 responses
11 views
0 likes
Last Post seqadmin  
Started by seqadmin, 11-30-2023, 10:48 AM
0 responses
17 views
0 likes
Last Post seqadmin  
Started by seqadmin, 11-29-2023, 08:26 AM
0 responses
14 views
0 likes
Last Post seqadmin  
Started by seqadmin, 11-29-2023, 08:12 AM
0 responses
14 views
0 likes
Last Post seqadmin  
Working...
X