Hi,
I wanted to calculate how many RefSeq genes were covered after QC in a particular Exome Seq project. First, all the bases above a certain coverage cutoff were extracted. Then I map those bases to transcripts of each gene. Now I have the number of bases covered for each gene. For one gene to be said "captured" in exome seq, how many bases should be covered, i.e. f one base of a gene is covered, should we say that gene was captured in exome seq? Overall, is it the right way to count covered genes?
Thanks in advance!
-
Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...09-07-2023, 11:15 PM -
Ribonucleic acid (RNA) represents a range of diverse molecules that play a crucial role in many cellular processes. From serving as a protein template to regulating genes, the complex processes involving RNA make it a focal point of study for many scientists. This article will spotlight various methods scientists have developed to investigate different RNA subtypes and the broader transcriptome.
Whole Transcriptome RNA-seq
Whole transcriptome sequencing...08-31-2023, 11:07 AM