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  • #1

    Evaluating overdispersion in CummeRbund

    Hi,

    I am analyzing RNA-Seq data in a TopHat/Cufflinks/CummeRbund pipeline. In the CummeRbund manual, it is recommended that researchers check for overdispersion for each sample as a quality control. How does one tell if a sample is overdispersed? Is there a statistical test that can quantify overdispersion?

    I have attached some of my dispersion plots for gene expression. Is there any way to tell if they are overdispersed?
    Attached Files
    Tags: cuffdiff, cummerbund, cummerbund plots, tophat
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