Hi friends,
I have 21 bp illumina RNA-seq tags samples across multiple tissues, and the reference genome sequence.
Is it Ok to use Tophat(which implement bowtie) and cufflink pipeline (http://www.nature.com/nprot/journal/....2012.016.html) to do differential expression analysis?
I have 21 bp illumina RNA-seq tags samples across multiple tissues, and the reference genome sequence.
Is it Ok to use Tophat(which implement bowtie) and cufflink pipeline (http://www.nature.com/nprot/journal/....2012.016.html) to do differential expression analysis?
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