Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Adapters trimming for BGI-seq?

    Hi everyone,

    Recently I just received PEreads from BGI-seq, and the sequencer company provide me with this adapters index:
    No code has to be inserted here........etc.....

    So, I used those shrt sequences to trim my reads using trimmomatic in Galaxy, and prepared the fasta seq as follow for trimming:

    >index1/1
    GGTCCATT
    >index1/2
    TTAGGTAG
    >index2/1
    GGTCCATT
    >index2/2
    GGTCACTA
    >index3/1
    GGTCCATT
    >index3/2
    TCCGCACT
    ...etc...

    Did I use the information correctly? because recently I just found that others have been using:
    forward_adapter = "AAGTCGGAGGCCAAGCGGTCTTAGGAAGACAA"
    reverse_adapter = "AAGTCGGATCGTAGCCATGTCGTTCTGTGAGCCAAGGAGTTG"

    to trim the adapters, not sure if its for SE only or also PE. Thank you

Latest Articles

Collapse

  • seqadmin
    Advanced Methods for the Detection of Infectious Disease
    by seqadmin




    The recent pandemic caused worldwide health, economic, and social disruptions with its reverberations still felt today. A key takeaway from this event is the need for accurate and accessible tools for detecting and tracking infectious diseases. Timely identification is essential for early intervention, managing outbreaks, and preventing their spread. This article reviews several valuable tools employed in the detection and surveillance of infectious diseases.
    ...
    11-27-2023, 01:15 PM
  • seqadmin
    Strategies for Investigating the Microbiome
    by seqadmin




    Microbiome research has led to the discovery of important connections to human and environmental health. Sequencing has become a core investigational tool in microbiome research, a subject that we covered during a recent webinar. Our expert speakers shared a number of advancements including improved experimental workflows, research involving transmission dynamics, and invaluable analysis resources. This article recaps their informative presentations, offering insights...
    11-09-2023, 07:02 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 09:55 AM
0 responses
11 views
0 likes
Last Post seqadmin  
Started by seqadmin, 11-30-2023, 10:48 AM
0 responses
17 views
0 likes
Last Post seqadmin  
Started by seqadmin, 11-29-2023, 08:26 AM
0 responses
14 views
0 likes
Last Post seqadmin  
Started by seqadmin, 11-29-2023, 08:12 AM
0 responses
14 views
0 likes
Last Post seqadmin  
Working...
X