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  • lkazen
    started a topic Trimmomatic error reading Fastq files

    Trimmomatic error reading Fastq files

    I'm trying to run Trimmomatic on some shotgun sequencing paired end files and while most of them are working (so I know the code isn't the problem), a handful of files are unable to be run. I ran this code:

    java -jar /scratch/g/jkirby/trimmomatic-0.39/trimmomatic-0.39.jar PE /scratch/g/jkirby/Landon_WGS_reanalysis/Figure_8/00_RAW_sequences/LTH2584Risp_108_R1.fastq.gz /scratch/g/jkirby/Landon_WGS_reanalysis/Figure_8/00_RAW_sequences/LTH2584Risp_108_R2.fastq.gz /scratch/g/jkirby/Landon_WGS_reanalysis/mouse_removal/LTH2584Risp_108_trimmed_R1.fastq.gz /scratch/g/jkirby/Landon_WGS_reanalysis/mouse_removal/LTH2584Risp_108_untrimmed_R1.fastq.gz /scratch/g/jkirby/Landon_WGS_reanalysis/mouse_removal/LTH2584Risp_108_trimmed_R2.fastq.gz /scratch/g/jkirby/Landon_WGS_reanalysis/mouse_removal/LTH2584Risp_108_untrimmed_R2.fastq.gz ILLUMINACLIP:TruSeq3-PE.fa:2:30:10:2:True LEADING:3 TRAILING:3 MINLEN:36

    And keep getting this error from Trimmomatic: Exception in thread "main" java.lang.RuntimeException: Invalid FASTQ name line: followed by a bunch of dark symbols with question marks inside for several hundred (or possibly several thousand) lines.

    A second file resulted in a similar error but the problematic line was completely different:

    Exception in thread "main" java.lang.RuntimeException: Invalid FASTQ comment line: GCCTCAGCTCCGGGATGTGCTTCATCAGATGGATAAAGGAATTTAGCCAAAGGCTACTTTTGTGGTGAACAGCGGGACAGGGTTGCATTTGAATTATGTCCAGACTCAGCCGGTTCCCATCTACACGCAGAACCAGCACTATCTGAAGGAG

    Clearly the error is with the fastq files but I'm not sure if there's a way to fix them?
    Last edited by lkazen; 10-12-2024, 09:33 AM.

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