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Basic guide: http://en.wikibooks.org/wiki/Next_Ge..._%28NGS%29/RNA
TopHat/Cufflinks: http://www.nature.com/nprot/journal/....2012.016.html
If you are a biologist wanting to learn (enough) UNIX to use some of the programs above then part I of this guide: http://korflab.ucdavis.edu/Unix_and_...rl_v3.1.1.html
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Mapping RNA seq reads
Hello, I am new to this forum. I am writing this thread because I wanted to know what would be the best way to go about mapping RNA seq reads that I have to my reference gneome (hg 19 genome). I have 8 fastq files that I need to map. I am new to this mapping and need help.Tags: None
Latest Articles
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by seqadmin
Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...-
Channel: Articles
10-18-2024, 07:11 AM -
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by seqadmin
Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.
Nobel Prize for MicroRNA Discovery
This week,...-
Channel: Articles
10-07-2024, 08:07 AM -
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New Model Aims to Explain Polygenic Diseases by Connecting Genomic Mutations and Regulatory Networks
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Started by seqadmin, 10-30-2024, 05:31 AM
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10-30-2024, 05:31 AM
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Started by seqadmin, 10-24-2024, 06:58 AM
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10-24-2024, 06:58 AM
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New AI Model Designs Synthetic DNA Switches for Targeted Gene Expression in Specific Cell Types
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Started by seqadmin, 10-23-2024, 08:43 AM
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10-23-2024, 08:43 AM
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