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I have a chip-seq sample where a significant proportion of the reads have low quality bases at the end. Trimming these bases using sickle or Trimmomatic produces reads of unequal length. Is this a problem for macs? I see that one of the first things macs does is to estimate the tag size based on the first 10 tags in the sample which may - or may not - be representative. Is macs assuming equal length for all tags?
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Like all molecular biology applications, next-generation sequencing (NGS) workflows require diligent quality control (QC) measures to ensure accurate and reproducible results. Proper QC begins at nucleic acid extraction and continues all the way through to data analysis. This article outlines the key QC steps in an NGS workflow, along with the commonly used tools and techniques.
Nucleic Acid Quality Control
Preparing for NGS starts with isolating the...02-10-2025, 01:58 PM -
In recent years, precision medicine has become a major focus for researchers and healthcare professionals. This approach offers personalized treatment and wellness plans by utilizing insights from each person's unique biology and lifestyle to deliver more effective care. Its advancement relies on innovative technologies that enable a deeper understanding of individual variability. In a joint documentary with our colleagues at Biocompare, we examined the foundational principles of precision...01-27-2025, 07:46 AM
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