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No, it is not a problem. It is used to check if the estimated fragment length differs from the read length.
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Chip-seq tags of unequal length - is this a problem for macs?
I have a chip-seq sample where a significant proportion of the reads have low quality bases at the end. Trimming these bases using sickle or Trimmomatic produces reads of unequal length. Is this a problem for macs? I see that one of the first things macs does is to estimate the tag size based on the first 10 tags in the sample which may - or may not - be representative. Is macs assuming equal length for all tags?
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by seqadmin
Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...-
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10-18-2024, 07:11 AM -
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by seqadmin
Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.
Nobel Prize for MicroRNA Discovery
This week,...-
Channel: Articles
10-07-2024, 08:07 AM -
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Yesterday, 06:09 AM
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10-30-2024, 05:31 AM
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10-24-2024, 06:58 AM
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Started by seqadmin, 10-23-2024, 08:43 AM
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10-23-2024, 08:43 AM
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