Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • no qcovs output from standalone blast

    I am having trouble getting query coverage output from local/standalone blast using qcovs:

    blastn -task megablast -db nt -query seqs.fasta -max_target_seqs 5 -outfmt "7 std qcovs"

    All the other outputs seem to be fine, but I cannot get query coverage output:

    # BLASTN 2.2.26+
    # Query: comp2_c0_seq1 len=213 path=[905:0-58 1657:59-212]
    # Database: /nfs/ffeng-lab-data/blastDBs/nt
    # Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, q. start, q. end, s. start, s. end, evalue, bit score
    # 5 hits found
    comp2_c0_seq1 gi|325197263|gb|AC241982.2| 94.76 210 11 0 1210 1947 1738 2e-86 327
    comp2_c0_seq1 gi|212374812|gb|AC217674.3| 94.29 210 12 0 1210 27303 27094 8e-85 322
    comp2_c0_seq1 gi|209915647|gb|AC194652.4| 94.29 210 12 0 1210 134086 133877 8e-85 322
    comp2_c0_seq1 gi|156255299|gb|AC199643.3| 94.29 210 12 0 1210 52769 52560 8e-85 322
    comp2_c0_seq1 gi|155029613|gb|AC199644.3| 94.29 210 12 0 1210 91737 91528 8e-85 322


    Any suggesions? thanks

  • #2
    BLAST 2.2.28+ was the first release with support for custom tabular output with query coverage.

    edit. 2.2.29+ was apparently released a few days ago
    Last edited by rhinoceros; 01-20-2014, 11:49 AM.
    savetherhino.org

    Comment


    • #3
      that would make sense

      i will get the newer version

      thanks!

      Comment

      Latest Articles

      Collapse

      • seqadmin
        New Genomics Tools and Methods Shared at AGBT 2025
        by seqadmin


        This year’s Advances in Genome Biology and Technology (AGBT) General Meeting commemorated the 25th anniversary of the event at its original venue on Marco Island, Florida. While this year’s event didn’t include high-profile musical performances, the industry announcements and cutting-edge research still drew the attention of leading scientists.

        The Headliner
        The biggest announcement was Roche stepping back into the sequencing platform market. In the years since...
        03-03-2025, 01:39 PM
      • seqadmin
        Investigating the Gut Microbiome Through Diet and Spatial Biology
        by seqadmin




        The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...
        02-24-2025, 06:31 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 12:50 PM
      0 responses
      11 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-03-2025, 01:15 PM
      0 responses
      182 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 02-28-2025, 12:58 PM
      0 responses
      278 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 02-24-2025, 02:48 PM
      0 responses
      664 views
      0 likes
      Last Post seqadmin  
      Working...
      X