Originally posted by maubp
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Are you using the latest version, BLAST+ 2.2.29 currently?
If so, I suggest you email the BLAST team with a bug report (and ideally offer to provide your data as a test case),
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Originally posted by mastal View PostHave you tried expressing 0.1 as 1e-01 instead?
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blast evalue strange behaviour
hello, I am experiencing a strange behaviour of blastn with the option "evalue":
$ blastn -task blastn -query _p -db genome.fa -outfmt 6
snoRNA_CD33 Chromosome_5 76.85 108 19 5 4 109 2773492 2773389 4e-11 66.2
snoRNA_CD33 Chromosome_2 91.30 23 1 1 45 66 7139854 7139876 2.9 30.1
snoRNA_CD33 Chromosome_1 94.74 19 1 0 28 46 3318593 3318575 2.9 30.1
good, found a hit at 4e-11 evalue
$ blastn -task blastn -query _p -db genome.fa -outfmt 6 -evalue 1
snoRNA_CD33 Chromosome_5 76.85 108 19 5 4 109 2773492 2773389 4e-11 66.2
same hit setting the evalue threshold at 1, as expected
$ blastn -task blastn -query _p -db genome.fa -outfmt 6 -evalue 0.1
$
no result! why? 4e-11 is still less than 0.1....
any idea?Tags: None
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