Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • The difference between transcripts and isoforms

    Hi everyone, i got confused at the concept "transcripts" and "isoforms", in some paper they mentioned "transcripts", some paper mentioned "isoforms", some mentioned both, i thought they were the same, is that right? Sorry for this silly questions. Thank you very much!

  • #2
    An isoform is a specific splice variant of an expressed gene. I'd say that a transcript is too, but I think the word "transcript" tends to be used a lot more loosely than "isoform".

    Comment


    • #3
      An isoform is specifically one of many splice variants of a gene, while a transcript is an expressed sequence. The difference is that "isoform" implies that there are multiple isoforms, while transcript makes no such implication. For example, prokaryote genes are mostly not spliced, so referring to them as isoforms generally does not make sense. For eukaryote genes with conditionally-used exons, referring to the individual expressed sequences as isoforms is useful.

      Comment


      • #4
        "The difference is that "isoform" implies that there are multiple isoforms, while transcript makes no such implication"

        Hi Brian, As you mentioned, i think isoform means there are alternative splicing, is that right? and for transcript can i suppose the expressed transcript is an expressed gene if this transcript has no isoform?

        Comment


        • #5
          Originally posted by crayfish View Post
          "The difference is that "isoform" implies that there are multiple isoforms, while transcript makes no such implication"

          Hi Brian, As you mentioned, i think isoform means there are alternative splicing, is that right? and for transcript can i suppose the expressed transcript is an expressed gene if this transcript has no isoform?
          It depends on your source and the paper you are reading, but I don't think you can assume that something referred to as a "transcript" has no alternative isoforms (unless it's a prokaryote). On the other hand, you can assume that something referred to as an isoform does have alternative isoforms.

          That said, some bioinformatics pipelines (eg trinity) will automatically pick both "genes" and "isoforms" and in this case, the program can refer to something as an "isoform" even if it has only picked one "isoform".
          Last edited by danwiththeplan; 04-29-2014, 08:47 PM.

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Genetic Variation in Immunogenetics and Antibody Diversity
            by seqadmin



            The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...
            Today, 07:24 PM
          • seqadmin
            Choosing Between NGS and qPCR
            by seqadmin



            Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...
            10-18-2024, 07:11 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, 11-01-2024, 06:09 AM
          0 responses
          24 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 10-30-2024, 05:31 AM
          0 responses
          21 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 10-24-2024, 06:58 AM
          0 responses
          25 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 10-23-2024, 08:43 AM
          0 responses
          55 views
          0 likes
          Last Post seqadmin  
          Working...
          X