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  • luc
    replied
    Razer S3 looks appropriate:

    Leave a comment:


  • ethanb416
    replied
    Thanks bioinfosm! I'll look into that. Do you have any experience with stampy or novoalign?

    Leave a comment:


  • bioinfosm
    replied
    i think you should change the query to aligner for multiply mapped reads!
    most aligners you mention have different options to select multiply mapped reads, so it might end up being multiple options from the same tool as well...

    Leave a comment:


  • ethanb416
    started a topic Best Aligner for Duplicated Sequences

    Best Aligner for Duplicated Sequences

    I'm looking around for some input from fellow SEQers. I'm experimenting with different aligners. I'm looking for an aligner that does a good job of correctly mapping reads from duplicated sequences, while at the same time is used fairly commonly. I am already looking into bwa, bowtie, and snap, but I would like to add one more to the list. Any suggestions? Would novoalign or stampy be good choices?

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  • seqadmin
    Addressing Off-Target Effects in CRISPR Technologies
    by seqadmin






    The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...
    08-27-2024, 04:44 AM

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