I am participating in the MinION access programme, and noticed that Nick Loman recorded a Nanopore run last night, using the most recent v7.3 flow cells. You can see the final read length histogram here:
[or watch from the start to see the entire 2h run]
This run produced a little under one read per second, with average read length about 7kbp, maximum sequence length of about 68kbp, producing about 45 Mbp over the course of the run. These numbers are estimated from the event data produced by the software, and the number of mappable bases will be less than this.
To reduce confusion slightly, the histogram shown in the MinKNOW program here is adjusted so that the bin height displays the summed length of sequences in each bin, rather than the number of sequences. As an example of interpretation for this run, the most base pairs were generated from sequences that were about 20kbp long.
-
Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...09-07-2023, 11:15 PM -
Ribonucleic acid (RNA) represents a range of diverse molecules that play a crucial role in many cellular processes. From serving as a protein template to regulating genes, the complex processes involving RNA make it a focal point of study for many scientists. This article will spotlight various methods scientists have developed to investigate different RNA subtypes and the broader transcriptome.
Whole Transcriptome RNA-seq
Whole transcriptome sequencing...08-31-2023, 11:07 AM