Hi everyone!
I got trapped in the following situation: I have some pairs of transcripts obtained by RNA-seq that were annotated with same terms, e.g. contigs A and B were annotated as 2-alkenal reductase (nadp(+)-dependent).
When I blasted them against genomes (blastn), refseq_rna (blastn) or refseq_protein (blastx), it seems that these two transcripts are fragments of a larger transcript (please see the attached files). Is this possible in a RNA-seq? Or maybe these transcripts are just isoforms?
Transcripts were assembled by a de novo technique since the organism of interest lacks a sequenced genome.
Best,
Marcio
I got trapped in the following situation: I have some pairs of transcripts obtained by RNA-seq that were annotated with same terms, e.g. contigs A and B were annotated as 2-alkenal reductase (nadp(+)-dependent).
When I blasted them against genomes (blastn), refseq_rna (blastn) or refseq_protein (blastx), it seems that these two transcripts are fragments of a larger transcript (please see the attached files). Is this possible in a RNA-seq? Or maybe these transcripts are just isoforms?
Transcripts were assembled by a de novo technique since the organism of interest lacks a sequenced genome.
Best,
Marcio
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