I am attempting to use cuffdiff to do a comparison analysis of various yeast transcriptome samples. I have processed the reads via Tophat and compiled transcripts via Cufflinks. I would like to use Cuffdiff to generate a comparison between samples so that I can start compiling which genes are differentially expressed. I have attempted to run Cuffdiff several times, but I keep getting the same "segmentation fault" error. Here is an example of my input:
$ cuffdiff ../ethanol/transcripts.gtf ~/RNA_Seq/Yeast/reads_for_comp/tophat_output/ethanol/accepted_hits_e.sam ~/RNA_Seq/Yeast/reads_for_comp/tophat_output/glucose/accepted_hits_g.sam
I quickly get the following output:
Segmentation fault (core dumped)
Does anyone have any experience with this type of error arising from Cuffdiff, and any suggestions as to how I can correct this?
Thanks in advance for your help.
$ cuffdiff ../ethanol/transcripts.gtf ~/RNA_Seq/Yeast/reads_for_comp/tophat_output/ethanol/accepted_hits_e.sam ~/RNA_Seq/Yeast/reads_for_comp/tophat_output/glucose/accepted_hits_g.sam
I quickly get the following output:
Segmentation fault (core dumped)
Does anyone have any experience with this type of error arising from Cuffdiff, and any suggestions as to how I can correct this?
Thanks in advance for your help.
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