When I use primer3+ to do the primer design. It gives out a warning:
Checking for poly-A and poly-T regions >= 7 bp, and potential hairpins ( >= 85% GC in a 30 bp window):
Amplimer 1: Poly-A region in amplimer TTGGAGGAAGATGCTTAGCC[...]GGTTAATGAAGTGTGGAGACTCC
What does this mean? Thanks
Checking for poly-A and poly-T regions >= 7 bp, and potential hairpins ( >= 85% GC in a 30 bp window):
Amplimer 1: Poly-A region in amplimer TTGGAGGAAGATGCTTAGCC[...]GGTTAATGAAGTGTGGAGACTCC
What does this mean? Thanks