Hello everybody!
I'm trying to analyse some pyrosequencing data that some colleges sent me. I could get all experiment replicas for each experimental condition. The problem is that for the different genes I can have a different number of replicas. Even more, in some cases some results are missing. I think i should refill those values with 0's or N/A. what should i do in those cases that i do not have value? Working with averages do not provides good statistics
example of data
HEK293-Nach A2 64.09 - 62.06 64.2 -
HEK293-Nach B2 69.97 - 69.3 71.36 -
HEK293-Nach B2 62.22 - 62.26 61.89 -
HEK293-Nach B3 71.59 - 71.64 68.18 -
HEK293-Nach B3 57.61 - 59.55 60.71 -
HEK293-Nach B4 58.39 - 60.47 58.35 -
HEK293-Nach B4 66.21 - 65.46 66.13 -
HEK293-Nach B5 54.93 - 57.03 57.47 -
HEK293-Nach B5 61.58 - 66.07 65.93 -
HEK293-Nach B6 50 - 56.69 55.01 -
HEK293-Nach B6 58.57 - 65.68 63.94 -
HEK293 K 45.1 46.4 42.15 1.9 41.37
HEK293 K 45.92 44.88 42.2 1.74 41.16
HEK293 K 62.49 67.06 59.34 2.35 58.34
HEK293 K 59.21 67.21 58.54 3.75 56.7
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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