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Analysis data with edgeR having some values missed

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  • paumarc
    started a topic Analysis data with edgeR having some values missed

    Analysis data with edgeR having some values missed

    Hello everybody!

    I'm trying to analyse some pyrosequencing data that some colleges sent me. I could get all experiment replicas for each experimental condition. The problem is that for the different genes I can have a different number of replicas. Even more, in some cases some results are missing. I think i should refill those values with 0's or N/A. what should i do in those cases that i do not have value? Working with averages do not provides good statistics

    example of data

    HEK293-Nach A2 64.09 - 62.06 64.2 -
    HEK293-Nach B2 69.97 - 69.3 71.36 -
    HEK293-Nach B2 62.22 - 62.26 61.89 -
    HEK293-Nach B3 71.59 - 71.64 68.18 -
    HEK293-Nach B3 57.61 - 59.55 60.71 -
    HEK293-Nach B4 58.39 - 60.47 58.35 -
    HEK293-Nach B4 66.21 - 65.46 66.13 -
    HEK293-Nach B5 54.93 - 57.03 57.47 -
    HEK293-Nach B5 61.58 - 66.07 65.93 -
    HEK293-Nach B6 50 - 56.69 55.01 -
    HEK293-Nach B6 58.57 - 65.68 63.94 -
    HEK293 K 45.1 46.4 42.15 1.9 41.37
    HEK293 K 45.92 44.88 42.2 1.74 41.16
    HEK293 K 62.49 67.06 59.34 2.35 58.34
    HEK293 K 59.21 67.21 58.54 3.75 56.7

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