Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • GenoMax
    replied
    As long as other things remain constant (no of cycles, sequencing kit version, machine) it should be ok.

    If it has been a while since you ran the first batch .... You may be able to leverage new technology (e.g. V2 rapid kits) and get all new data that can give you the coverage you desire.

    Leave a comment:


  • adrian
    replied
    Any suggestions I appreciate..
    thanks

    Leave a comment:


  • Merging RNA-Seq files run at two different times with identical setup

    Hi group:
    I am sure this question has been asked in many flavors but after pouring over those, I still could not get what exactly I am asking for. I thought members could provide an answer.

    My question:

    I have two samples with 3 RNA-Seq replicates ( by combining all 3 replicates from two lanes I get 30Mil reads 100bp length PE reads).

    Obviously this is not a good read coverage for identifying splicing differences.

    I am aiming now for 70Mil reads to identify the splicing differences.

    My question:
    1. I am going for another sequencing run shooting for another 40 Mil read coverage.

    Can I combine the new 40 Mil reads with previous 30 Mil reads and do analysis for identifying splicing patterns. Is there any hidden surprises that one cannot combine two batches like these (concerning insert sizes etc..). I will use MATS for splicing.

    Obviously I will choose the same design (100bp and paired-end design) and I have the same RNA library left from previous sequencing run which I will use for new sequencing.


    thanks a lot.
    Adrian

Latest Articles

Collapse

  • seqadmin
    Genetic Variation in Immunogenetics and Antibody Diversity
    by seqadmin



    The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...
    11-06-2024, 07:24 PM
  • seqadmin
    Choosing Between NGS and qPCR
    by seqadmin



    Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...
    10-18-2024, 07:11 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 11:09 AM
0 responses
24 views
0 likes
Last Post seqadmin  
Started by seqadmin, Today, 06:13 AM
0 responses
23 views
0 likes
Last Post seqadmin  
Started by seqadmin, 11-01-2024, 06:09 AM
0 responses
31 views
0 likes
Last Post seqadmin  
Started by seqadmin, 10-30-2024, 05:31 AM
0 responses
21 views
0 likes
Last Post seqadmin  
Working...
X