I have a reference genome (.fa) and its annotation (.gtf) files. I have sequenced both DNA and RNA from test sample and generated vcf files containing SNP and INDEL locations for each chromosome of the reference genome. I want to plot the identified SNPs and INDELs to identify hot spots on each chromosome. I have read about R being useful to do the same but was unable to narrow down on how to get it done. Please advise.......
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by seqadmin
The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...-
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11-06-2024, 07:24 PM -
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by seqadmin
Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...-
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10-18-2024, 07:11 AM -
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