Announcement

Collapse
No announcement yet.
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • #1

    How to get bwa to report one alignment per read or read pair

    I'm using bwa with the parameters 'sampe -n 1 -N 0' to report 1 alignment per read for a paired-end dataset. However, in the output sam file, I see the same forward/reverse pair report twice to different locations:

    HWI:1:X:2:1101:10063:24879 117 chr1 242612515 0 * = 242612515 0
    HWI:1:X:2:1101:10063:24879 153 chr1 242612515 37 91M = 242612515 0
    HWI:1:X:2:1101:10063:24879 163 chr7 14028507 60 100M = 14028669 264
    HWI:1:X:2:1101:10063:24879 83 chr7 14028669 60 39M1D62M = 14028507 -264

    Why would this happen?
    Tags: None
Previous template Next

Latest Articles

Collapse
  • seqadmin
    Advanced Methods for the Detection of Infectious Disease
    by seqadmin




    The recent pandemic caused worldwide health, economic, and social disruptions with its reverberations still felt today. A key takeaway from this event is the need for accurate and accessible tools for detecting and tracking infectious diseases. Timely identification is essential for early intervention, managing outbreaks, and preventing their spread. This article reviews several valuable tools employed in the detection and surveillance of infectious diseases.
    ...
    11-27-2023, 01:15 PM
  • seqadmin
    Strategies for Investigating the Microbiome
    by seqadmin




    Microbiome research has led to the discovery of important connections to human and environmental health. Sequencing has become a core investigational tool in microbiome research, a subject that we covered during a recent webinar. Our expert speakers shared a number of advancements including improved experimental workflows, research involving transmission dynamics, and invaluable analysis resources. This article recaps their informative presentations, offering insights...
    11-09-2023, 07:02 AM
View All

ad_right_rmr

Collapse

News

Collapse
Topics Statistics Last Post
Single-Cell Sequencing: A New Era in Understanding Genetic Developmental Disorders by seqadmin
Started by seqadmin, 12-01-2023, 09:55 AM
0 responses
15 views
0 likes
 Last Post seqadmin
by seqadmin
12-01-2023, 09:55 AM  
UK Biobank Releases Largest Single Set of Whole Genome Sequencing Data by seqadmin
Started by seqadmin, 11-30-2023, 10:48 AM
0 responses
19 views
0 likes
 Last Post seqadmin
by seqadmin
11-30-2023, 10:48 AM  
Advancing Personalized Medicine with AI: A New Approach to Cell Analysis by seqadmin
Started by seqadmin, 11-29-2023, 08:26 AM
0 responses
14 views
0 likes
 Last Post seqadmin
by seqadmin
11-29-2023, 08:26 AM  
Exploring Genetic Mysteries: DNA Methylation's Role in Disease Variation by seqadmin
Started by seqadmin, 11-29-2023, 08:12 AM
0 responses
15 views
0 likes
 Last Post seqadmin
by seqadmin
11-29-2023, 08:12 AM  
View All
Working...
X