Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • how to set one sample as a control for my other sample in gene differential analysis

    Hello everyone
    I have a question.I have 3 stages of 2 diff genotype from a single tissue type i would like to chk differential gene expression analysis among these genotype at different stage. but to chk the gene that are specifically expressed in this particular tissue we also collect sample from one another tissue that may be vegetative or some other (it can be a used as a control for different stages ). so that we get to know what are the specific gene for this tissue. I would like to know how to compare or set other tissue as a reference for my tissue type.
    how to set one sample as a control against other sample in gene differential analysis
    can you suggest something on it ?
    Last edited by meghavarshney; 08-11-2015, 01:14 AM.

  • #2
    While you can compare the raw expression between the two phenotypes at the transcript/gene level, you will not be able to assess any kind of statically significant meaning (i.e. no p-value). It is generally recommended to have at least 3 samples of each phenotype you are trying to compare in order to generate/calculate a distribution and assess the likelihood of obtaining the measurement by random chance alone.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      The Impact of AI in Genomic Medicine
      by seqadmin



      Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
      02-26-2024, 02:07 PM
    • seqadmin
      Multiomics Techniques Advancing Disease Research
      by seqadmin


      New and advanced multiomics tools and technologies have opened new avenues of research and markedly enhanced various disciplines such as disease research and precision medicine1. The practice of merging diverse data from various ‘omes increasingly provides a more holistic understanding of biological systems. As Maddison Masaeli, Co-Founder and CEO at Deepcell, aptly noted, “You can't explain biology in its complex form with one modality.”

      A major leap in the field has
      ...
      02-08-2024, 06:33 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Today, 06:12 AM
    0 responses
    13 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 02-23-2024, 04:11 PM
    0 responses
    67 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 02-21-2024, 08:52 AM
    0 responses
    70 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 02-20-2024, 08:57 AM
    0 responses
    61 views
    0 likes
    Last Post seqadmin  
    Working...
    X