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  • apredeus
    started a topic PCA and DE analysis for loop design microarray

    PCA and DE analysis for loop design microarray

    Hi all,

    I am working on a piece of data of a kind I've never dealt with before, and I'd like your input if anybody has experience dealing with things like this. I have an Agilent two-color microarray with 8 sub-arrays (1_1.. 1_4, 2_1..2_4) that involve 3 conditions (Control, Delta, NS). Biological replicas were combined into one pool each (don't ask), so there's basically only technical variation to assess.

    If you can help me with suggestions how can I analyze it I would appreciate it. Here's is the design scheme:

    Last edited by apredeus; 09-08-2015, 06:16 AM.

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  • seqadmin
    Current Approaches to Protein Sequencing
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    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
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