Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • GenoMax
    replied
    If you actually have data in "tables" then perhaps you only have a reduced representation of the data (unless you are referring to a VCF file is tab-delimited).

    If that is the case then you could use vcftools/bedtools "intersect" to find the SNP's that are common in both files. All caveats mentioned by @blancha still apply.

    Hopefully you know "what data is what" and are only planning to match the SNP's as additional QC confirmation for the samples.

    Leave a comment:


  • blancha
    replied
    Your question is difficult to understand, but I am assuming you are trying to compare SNPs found in RNA-Seq data to SNPs found in DNA-Seq data.

    For individual genes and SNPs, you could compare the datasets visually by loading the BAM files or the VCF files in IGV.

    For a genome wide comparison, you can just compare the mutations in the VCF files or the data from the VCF files converted to an Excel file.
    There has to be some manual curation, and cutoffs set for the RNA-Seq data. The coverage will be uneven for the RNA-Seq data, and, amongst other issues, indels may not be handled properly.

    Leave a comment:


  • How to find same sequences between two sequencing project

    Hello, I have two tables of sequences, one from DNA seq, one from RNA seq. Is there a easy way for me to it find the same sequences in both tables?


    I used the same individuals for DNA seq and RNA seq. Now I want to match the individuals using same SNPs in both project.

    Thanks for the help.

Latest Articles

Collapse

  • seqadmin
    Recent Advances in Sequencing Technologies
    by seqadmin







    Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.

    Long-Read Sequencing
    Long-read sequencing has...
    Today, 01:49 PM
  • seqadmin
    Genetic Variation in Immunogenetics and Antibody Diversity
    by seqadmin



    The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...
    11-06-2024, 07:24 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 09:29 AM
0 responses
13 views
0 likes
Last Post seqadmin  
Started by seqadmin, Today, 09:06 AM
0 responses
11 views
0 likes
Last Post seqadmin  
Started by seqadmin, Today, 08:03 AM
0 responses
11 views
0 likes
Last Post seqadmin  
Started by seqadmin, 11-22-2024, 07:36 AM
0 responses
61 views
0 likes
Last Post seqadmin  
Working...
X