Hi,
I am running the fastqc (v0.11.4) on Ubuntu 14.04.3 LTS.
I have four fastq files (two pairs of paired-end reads samples). They are AFAIK from old solexa machines in sanger format
somehow when I am trying to fastq the _1 files I get the following error message:
than nothing happens.
When I am using the same command for the _2 files, it works fine.
I can't see any differences in the format of the two files.
the header of the two files from one of the pairs looks like that:
and
Any ideas, why I can't run the _1 files?
thanks
Assa
P.S.
When I am running the SolexaQA++ tool, I can read all the four files without difficulties.
I am running the fastqc (v0.11.4) on Ubuntu 14.04.3 LTS.
I have four fastq files (two pairs of paired-end reads samples). They are AFAIK from old solexa machines in sanger format
somehow when I am trying to fastq the _1 files I get the following error message:
Code:
fastqc -t 12 -o ../Results/1c3c603f-29ac-4263-851d-b19f9ce4cfb0/fastqcResults/ 61627AAXX_1_1.fastq.gz Started analysis of 61627AAXX_1_1.fastq.gz Exception in thread "Thread-1" java.lang.IllegalArgumentException: Unexpected cs char C at uk.ac.babraham.FastQC.Sequence.FastQFile.convertColorspaceToBases(FastQFile.java:334) at uk.ac.babraham.FastQC.Sequence.FastQFile.readNext(FastQFile.java:191) at uk.ac.babraham.FastQC.Sequence.FastQFile.next(FastQFile.java:125) at uk.ac.babraham.FastQC.Analysis.AnalysisRunner.run(AnalysisRunner.java:76) at java.lang.Thread.run(Thread.java:745)
When I am using the same command for the _2 files, it works fine.
Code:
fastqc -t 12 -o ../Results/1c3c603f-29ac-4263-851d-b19f9ce4cfb0/fastqcResults/ 61627AAXX_1_2.fastq.gz Started analysis of 61627AAXX_1_2.fastq.gz Approx 5% complete for 61627AAXX_1_2.fastq.gz ...
the header of the two files from one of the pairs looks like that:
Code:
zcat 61GAFAAXX_1_1.fastq.gz | head -n 12 @SOLEXA12_1:1:1:990:4777/1 1:Y:0:0 .................................................. + ################################################## @SOLEXA12_1:1:1:990:11674/1 1:Y:0:0 .................................................. + ################################################## @SOLEXA12_1:1:1:990:17662/1 1:Y:0:0 .................................................. + ##################################################
Code:
zcat 61GAFAAXX_1_2.fastq.gz | head -n 12 @SOLEXA12_1:1:1:990:4777/2 2:Y:0:0 .................................................. + ################################################## @SOLEXA12_1:1:1:990:11674/2 2:Y:0:0 .................................................. + ################################################## @SOLEXA12_1:1:1:990:17662/2 2:Y:0:0 .................................................. + ##################################################
thanks
Assa
P.S.
When I am running the SolexaQA++ tool, I can read all the four files without difficulties.
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