Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • qtrinh
    replied
    Here is a link that describes the output of consensus and indels calling: http://samtools.sourceforge.net/cns0.shtml

    Leave a comment:


  • Mansequencer
    started a topic samtools pileup output for SNP detection

    samtools pileup output for SNP detection

    Hi All,
    I aligned 50 bp solexa reads onto a reference genome using bowtie and now I am using samtools pileup -vcf to call SNPs. This is the output it generates:
    scaffold179 29989 T A 30 30 60 1 A B
    scaffold179 29991 A G 4 4 60 1 G #
    scaffold179 29992 A T 4 4 60 1 T #
    scaffold179 29993 T A 4 4 60 1 N #
    scaffold179 29994 T A 4 4 60 1 N #
    scaffold179 29995 A C 4 4 60 1 C #
    scaffold179 29997 T A 4 4 60 1 N #
    scaffold179 29998 G C 4 4 60 1 C #
    scaffold179 29999 T C 4 4 60 1 C #
    scaffold179 30000 T A 4 4 60 1 N #
    scaffold179 30001 T A 4 4 60 1 A #
    scaffold179 30002 G A 4 4 60 1 A #
    scaffold179 30003 A G 4 4 60 1 G #
    scaffold179 30004 T C 4 4 60 1 C #
    scaffold179 30005 T A 4 4 60 1 A #
    scaffold179 30006 T A 4 4 60 1 N #
    scaffold179 30007 G A 4 4 60 1 N #
    scaffold179 30008 T A 4 4 60 1 N #
    scaffold179 30009 G A 4 4 60 1 N #
    scaffold179 30010 T A 4 4 60 1 N #
    scaffold179 30011 T A 4 4 60 1 N #
    scaffold179 30012 T A 4 4 60 1 N #
    scaffold179 30013 T A 4 4 60 1 N #
    scaffold179 30014 G A 4 4 60 1 N #

    I understand that columns from left to right are:
    Scaffold name:
    coordinate:
    reference base:
    polymorphic base:

    I am confused about the numbers after the 4th column. Please suggest as to what they correspond to.
    Also when I try to look at the alignment in tview, it shows only Ns after the first 80 pb. My reference genome does contain some short stretches of Ns but is not all Ns so why does the samtools tview shows only Ns?
    Any suggestion would be highly appreciated.
    Thanks for all the help.
    Thanks.

Latest Articles

Collapse

  • seqadmin
    Quality Control Essentials for Next-Generation Sequencing Workflows
    by seqadmin




    Like all molecular biology applications, next-generation sequencing (NGS) workflows require diligent quality control (QC) measures to ensure accurate and reproducible results. Proper QC begins at nucleic acid extraction and continues all the way through to data analysis. This article outlines the key QC steps in an NGS workflow, along with the commonly used tools and techniques.

    Nucleic Acid Quality Control
    Preparing for NGS starts with isolating the...
    02-10-2025, 01:58 PM
  • seqadmin
    An Introduction to the Technologies Transforming Precision Medicine
    by seqadmin


    In recent years, precision medicine has become a major focus for researchers and healthcare professionals. This approach offers personalized treatment and wellness plans by utilizing insights from each person's unique biology and lifestyle to deliver more effective care. Its advancement relies on innovative technologies that enable a deeper understanding of individual variability. In a joint documentary with our colleagues at Biocompare, we examined the foundational principles of precision...
    01-27-2025, 07:46 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 02-07-2025, 09:30 AM
0 responses
72 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-05-2025, 10:34 AM
0 responses
113 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-03-2025, 09:07 AM
0 responses
87 views
0 likes
Last Post seqadmin  
Started by seqadmin, 01-31-2025, 08:31 AM
0 responses
48 views
0 likes
Last Post seqadmin  
Working...
X