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RSEM needs alignments against the transcriptome, so it'd be a big problem if you had chromosomal coordinates. So this is expected (required even), it's not a problem.Leave a comment:
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RSEM bam question
Hi, I used RSEM with STAR. I noticed that in the RNAME field of the BAM (3rd filed) , where usually had e.g chr1 now I have the name of the isoform. With this BAM I can not find the chr but I need this information.
Is it normal to have this kind of BAM?
How can I solve this problem?
Thank you.
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Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
Long-read sequencing has seen remarkable advancements,...12-02-2024, 01:49 PM
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