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Hello All,
I am working with samples that have miRNA/tRNA and piRNA. Is there a sane way to normalize this data for sequencing depth bias?
Simple count standardization to a million mapped tags doesn't seem sufficient, but I'm unsure of a better way to approach this. We did spike-in with a c-elegans miRNA spike in, but is it appropriate to standardize tRNA populations using that miRNA?
Any recommendations would be greatly appreciated!
I am working with samples that have miRNA/tRNA and piRNA. Is there a sane way to normalize this data for sequencing depth bias?
Simple count standardization to a million mapped tags doesn't seem sufficient, but I'm unsure of a better way to approach this. We did spike-in with a c-elegans miRNA spike in, but is it appropriate to standardize tRNA populations using that miRNA?
Any recommendations would be greatly appreciated!