Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
It appears that bowtie do not print out U nucleotides. However, could it be the problem of low mapping percentage that input is sequence data (that is cDNA) and reference is mature miRNA?
-
Problem with Bowtie alignment
Novice here. I tried to align miRNA sequencing data (Illumina) with mature miRNA from mirbase.org database using Bowtie. Reads are adapter trimmed and mapped to human genome hg19 by someone else. Length is about 26 nt. However, i get very small portion of aligned reads to mature miRNAs (less than 1%). I have tried options: -n 0 -l 20 (-l 17 - no difference). However, even those reads that got aligned were weird because of the length of the aligned reads in an output.
I then experimented with some mirna sequences to see how do results look in a more controlled manner: i aligned sequence from reference genome (mature miRNAs) with a indexed mature miRNAs reference. Of course, it got aligned but in a output it wrote some 13 nucleotide sequence that was not in input.
Input was 22 nt:
>hsa-let-7a-5p MIMAT0000062 Homo sapiens let-7a-5p
UGAGGUAGUAGGUUGUAUAGUU.
Output was 13 nt:
hsa-let-7a-5p MIMAT0000062 Homo sapiens let-7a-5p + hsa-let-7a-5p 0 GAGGAGAGGGAAG IIIIIIIIIIIII 0
I would like to know what this output means. As far as i know the sequence should be the sequence that got aligned. If i trimm (-3 <int> or -5 <int) the 13 nt sequence shortens by a indicated number.
Latest Articles
Collapse
-
by seqadmin
Spatial biology is an exciting field that encompasses a wide range of techniques and technologies aimed at mapping the organization and interactions of various biomolecules in their native environments. As this area of research progresses, new tools and methodologies are being introduced, accompanied by efforts to establish benchmarking standards and drive technological innovation.
3D Genomics
While spatial biology often involves studying proteins and RNAs in their...-
Channel: Articles
01-01-2025, 07:30 PM -
-
by seqadmin
Many organizations study rare diseases, but few have a mission as impactful as Rady Children’s Institute for Genomic Medicine (RCIGM). “We are all about changing outcomes for children,” explained Dr. Stephen Kingsmore, President and CEO of the group. The institute’s initial goal was to provide rapid diagnoses for critically ill children and shorten their diagnostic odyssey, a term used to describe the long and arduous process it takes patients to obtain an accurate...-
Channel: Articles
12-16-2024, 07:57 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 01-09-2025, 04:04 PM
|
0 responses
12 views
0 likes
|
Last Post
by seqadmin
01-09-2025, 04:04 PM
|
||
Started by seqadmin, 01-09-2025, 09:42 AM
|
0 responses
20 views
0 likes
|
Last Post
by seqadmin
01-09-2025, 09:42 AM
|
||
Started by seqadmin, 01-08-2025, 03:17 PM
|
0 responses
29 views
0 likes
|
Last Post
by seqadmin
01-08-2025, 03:17 PM
|
||
Started by seqadmin, 01-03-2025, 11:18 AM
|
1 response
47 views
1 like
|
Last Post
by Tonia
01-05-2025, 12:15 PM
|
Leave a comment: