I am trying to align my rna-seq reads for circRNA discovery, but tophat is throwing error with the following message:
[2016-11-03 16:33:29] Searching for junctions via segment mapping
[FAILED]
Error: segment-based junction search failed with err =-6
Reason: image not found
Any help would be greatly appreciated!
[2016-11-03 16:33:29] Searching for junctions via segment mapping
[FAILED]
Error: segment-based junction search failed with err =-6
Reason: image not found
Any help would be greatly appreciated!