Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • p-value and fold change

    Hello,

    I got my rna-seq analysis from the company and they have assigned p-value to each gene. Is it possible to have a p-value from only one set of data since I had sent only one batch and there were no replicates? Also, some of my genes have p>0.05 (not differentially expressed) but high fold-change increase. So which of the two should be considered important - p-value or fold change?

    Thanks

  • #2
    If you only sent them single samples from your groups then the p-values are largely meaningless. There's not a direct correspondence between fold-change and p-value, since lowly expressed genes will randomly show higher fold-changes (i.e., the p-value is a function of expression level and fold change).

    Comment


    • #3
      Ok. But can the p-value be between my control and treatment samples as this is what they mentioned in their analysis and p<0.05 means those genes are differentially expressed?

      Thanks

      Comment


      • #4
        The presumption is that the p-value is from a poisson test, so yes it's between the two samples.

        Yes, p<0.05 would normally mean differentially expressed, aside from the fact that the p-values in your case are largely meaningless.

        Comment


        • #5
          So I guess the p-value is between technical replicates hence meaningless. Thanks a lot!

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Latest Developments in Precision Medicine
            by seqadmin



            Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

            Somatic Genomics
            “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
            Yesterday, 01:16 PM
          • seqadmin
            Recent Advances in Sequencing Analysis Tools
            by seqadmin


            The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
            05-06-2024, 07:48 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, Yesterday, 07:15 AM
          0 responses
          13 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 05-23-2024, 10:28 AM
          0 responses
          17 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 05-23-2024, 07:35 AM
          0 responses
          19 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 05-22-2024, 02:06 PM
          0 responses
          10 views
          0 likes
          Last Post seqadmin  
          Working...
          X