Announcement

Collapse

Welcome to the New Seqanswers!

Welcome to the new Seqanswers! We'd love your feedback, please post any you have to this topic: New Seqanswers Feedback.
See more
See less

Bimodal Distribution of Mismatch Frequencies in Illumina

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Bimodal Distribution of Mismatch Frequencies in Illumina

    I created a pileup file using Maq. If I look at the log transformed distributions of frequencies of mismatch for each nucleotide across all positions,

    (forward mismatch /forward depth + reverse mismatch/reverse depth)/2

    , I end up getting a bimodal distribution for each of the nucleotides accept one. I think 'A' looks more normally distributed but I can't remember for sure. Has anyone else seen this and do you have any idea what could be causing this?
    Last edited by mdjones66; 10-27-2010, 07:42 PM. Reason: grammer
Working...
X