I have a sets of Illumina pair-end data sets and used it as input file for de-novo genome assembler program.
I have generate corresponding assembly contigs and I plan to run SSPACE for scaffolding now.
I have 4 sets of Illumina mate pair library now:
PE100, Read length 100 nt, 10kb insert size, mate-pair (s_PE100_10kb_1.fastq);
PE100, Read length 100 nt, 10kb insert size, mate-pair (s_PE100_10kb_2.fastq);
PE100, Read length 100 nt, 5kb insert size, mate-pair (s_PE100_5kb_1.fastq);
PE100, Read length 100 nt, 5kb insert size, mate-pair (s_PE100_5kb_2.fastq);
PE35, Read length 35 nt, 10kb insert size, mate-pair (s_PE35_10kb_1.fastq);
PE35, Read length 35 nt, 10kb insert size, mate-pair (s_PE35_10kb_2.fastq);
PE35, Read length 35 nt, 5kb insert size, mate-pair (s_PE35_5kb_1.fastq);
PE35, Read length 35 nt, 5kb insert size, mate-pair (s_PE35_5kb_2.fastq);
My mate pair read is range from 35 - 100 nt length and insert size is 5kb - 10kb.
Can I know how I should arrange my library file for SSPACE_Standard_v3.0.pl?
I not too sure how to prepare library file when my mate pair library is different read length and different insert size.
Thanks for any advice.
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
Latest Articles
Collapse
-
by noor121Qadri offers efficient online services designed for students and staff of University Targu Mures Medical Campus Hamburg. We streamline your academic and administrative processes for a hassle-free experience.
VIsit us:
https://qadri-international.com/univ...s-hamburg-umch...-
Channel: Articles
Today, 09:33 PM -
-
by seqadmin
Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.
Nobel Prize for MicroRNA Discovery
This week,...-
Channel: Articles
Yesterday, 08:07 AM -
-
by seqadmin
Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...-
Channel: Articles
09-23-2024, 06:35 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 10-02-2024, 04:51 AM
|
0 responses
96 views
0 likes
|
Last Post
by seqadmin
10-02-2024, 04:51 AM
|
||
Started by seqadmin, 10-01-2024, 07:10 AM
|
0 responses
107 views
0 likes
|
Last Post
by seqadmin
10-01-2024, 07:10 AM
|
||
Started by seqadmin, 09-30-2024, 08:33 AM
|
1 response
107 views
0 likes
|
Last Post
by EmiTom
Yesterday, 06:46 AM
|
||
Started by seqadmin, 09-26-2024, 12:57 PM
|
0 responses
20 views
0 likes
|
Last Post
by seqadmin
09-26-2024, 12:57 PM
|