-
Probably, there is the problem with sorting. (check https://twitter.com/davisjmcc/status/931298649841188864 ) -
Sorry for the late followup to your reply. If that is the case, then why am I getting poorer quality when I convert the bam to 2 fastq files(read 1 and 2) whereas good quality when just checking the bam file as a whole?Leave a comment:
-
Yes, because FastQC is a QC program. FastQC does not modify raw data. It is going to show you summary of the same Q-scores whether they come from the BAM or the fastq reads extracted from the BAM file.Leave a comment:
-
Bam quality vs read quality
I have a cancer bam file and want to check its read qualities. I have two options in mind.
1. Check bam quality through fastqc
2. Convert the bam file into paired-end reads (in fastq) and check their quality in fastqc.
Are both the processes equivalent?
-
At the intersection of cytogenetics and genomics lies the exciting field of cytogenomics. It focuses on studying chromosomes at a molecular scale, involving techniques that analyze either the whole genome or particular DNA sequences to examine variations in structure and behavior at the chromosomal or subchromosomal level. By integrating cytogenetic techniques with genomic analysis, researchers can effectively investigate chromosomal abnormalities related to diseases, particularly...09-26-2023, 06:26 AM -
Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...09-07-2023, 11:15 PM
Leave a comment: