Announcement

Collapse
No announcement yet.
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • #1

    Unicycler

    I know Unicycler is specialised for bacterial genome assembling from hybrid of short and long reads.
    But, is Unicycler also good for bacterial genome assembling only from short reads such as from Illumina MiSeq
    Tags: None
  • #2
    Yes, because it acts as an optimiser for Spades.. which is perfectly happy with just short read data.

    Comment

    • #3
      Thak you very much!
      I have one set paired (2 x250) Illumnia MiSeq data from Salmonella.
      They look good by FastaQC
      I did Unicycler assembling but I did not get a whole genome.
      The longest contig is 446213 and totally I got 59 contigs longer than 500 bp.
      What can I do next?

      Comment

      • #4
        Some minION sequencing?

        There's nothing wrong with that number of contigs from a 2x250 MiSeq run.

        Comment

        Previous template Next

        Latest Articles

        Collapse
        • seqadmin
          Advanced Methods for the Detection of Infectious Disease
          by seqadmin




          The recent pandemic caused worldwide health, economic, and social disruptions with its reverberations still felt today. A key takeaway from this event is the need for accurate and accessible tools for detecting and tracking infectious diseases. Timely identification is essential for early intervention, managing outbreaks, and preventing their spread. This article reviews several valuable tools employed in the detection and surveillance of infectious diseases.
          ...
          11-27-2023, 01:15 PM
        • seqadmin
          Strategies for Investigating the Microbiome
          by seqadmin




          Microbiome research has led to the discovery of important connections to human and environmental health. Sequencing has become a core investigational tool in microbiome research, a subject that we covered during a recent webinar. Our expert speakers shared a number of advancements including improved experimental workflows, research involving transmission dynamics, and invaluable analysis resources. This article recaps their informative presentations, offering insights...
          11-09-2023, 07:02 AM
        View All

        ad_right_rmr

        Collapse

        News

        Collapse
        Topics Statistics Last Post
        Non-Invasive DNA Analysis: A New Tool for Monitoring Polar Bears by seqadmin
        Started by seqadmin, Yesterday, 02:24 PM
        0 responses
        11 views
        0 likes
        		 Last Post seqadmin
        by seqadmin
        Yesterday, 02:24 PM  
        Innovative Technique Enhances Understanding of Gene Regulation by seqadmin
        Started by seqadmin, Yesterday, 07:37 AM
        0 responses
        21 views
        0 likes
        		 Last Post seqadmin
        by seqadmin
        Yesterday, 07:37 AM  
        New Cultivars on the Horizon: Over 100 Magic Mushroom Genomes Analyzed by seqadmin
        Started by seqadmin, 12-04-2023, 08:23 AM
        0 responses
        8 views
        0 likes
        		 Last Post seqadmin
        by seqadmin
        12-04-2023, 08:23 AM  
        Single-Cell Sequencing: A New Era in Understanding Genetic Developmental Disorders by seqadmin
        Started by seqadmin, 12-01-2023, 09:55 AM
        0 responses
        24 views
        0 likes
        		 Last Post seqadmin
        by seqadmin
        12-01-2023, 09:55 AM  
        View All
        Working...
        X