Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Protein annotation parameters (query or subject coverage?)

    Hi there.

    I am annotating some proteins' functions and I a have some doubts about the parameters I should use. I'm using e-value below 1x10-4, identity above 40% and coverage above 70%, but I don't know if a sould use the query coverage or the subject coverage for annotation.
    I only saw the query coverage being used (the % of your query sequence that overlaps the subject sequence), but it makes more sense to me and some colleagues to use the subject coverage (the % of the subject sequence that overlaps your query sequence). Since the protein with the known function is the subject one, if you find most of it within the query coverage it means that your query protein has higher probability to share the subject protein's function.
    If some one could explain why I sould use the query or the subject coverage I would be very greateful (sorry if it is an obvious question).

Latest Articles

Collapse

  • seqadmin
    Latest Developments in Precision Medicine
    by seqadmin



    Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

    Somatic Genomics
    “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
    05-24-2024, 01:16 PM
  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    05-06-2024, 07:48 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 05-24-2024, 07:15 AM
0 responses
16 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-23-2024, 10:28 AM
0 responses
18 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-23-2024, 07:35 AM
0 responses
22 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-22-2024, 02:06 PM
0 responses
11 views
0 likes
Last Post seqadmin  
Working...
X