Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Pipelines for identifying subclonal mutational events in WGS with 25X coverage

    I have been trying to use PyClone (https://www.nature.com/articles/nmeth.2883#abstract) to do subclonal mutational analysis on whole genome sequencing data of cell lines, but because the coverage is only 25X, am having a hard time using PyClone.

    I set some read depth filters to decrease which mutations to analyze using PyClone because the pipeline was taking too long to run on the entire list of mutations called (~4.5 million).

    Are there any available pipelines for subclonality analysis on data with low coverage?

Latest Articles

Collapse

  • seqadmin
    Genetic Variation in Immunogenetics and Antibody Diversity
    by seqadmin



    The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...
    11-06-2024, 07:24 PM
  • seqadmin
    Choosing Between NGS and qPCR
    by seqadmin



    Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...
    10-18-2024, 07:11 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 11-08-2024, 11:09 AM
0 responses
31 views
0 likes
Last Post seqadmin  
Started by seqadmin, 11-08-2024, 06:13 AM
0 responses
26 views
0 likes
Last Post seqadmin  
Started by seqadmin, 11-01-2024, 06:09 AM
0 responses
32 views
0 likes
Last Post seqadmin  
Started by seqadmin, 10-30-2024, 05:31 AM
0 responses
22 views
0 likes
Last Post seqadmin  
Working...
X