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  • Need Help! Vsearch fastq_filter quality value above qmax

    Hello all!

    I'm new to the community and just started my first bioinformatics project. I'm filtering out merged reads with the expected error of 1-100.

    code I'm running:
    vsearch -fastq_filter ./MergedFiles/${otp}_merged.fastq -fastq_maxee 1 -fastqout ./MergedFiles/${otp}_mergedfilt.fastq -fastaout ./MergedFiles/${otp}_mergedfilt.fasta


    The issue is that when I run it, I get back a fatal error saying-

    Reading input file 0%
    Fatal error: FASTQ quality value (42) above qmax (41)


    I've tried usearch, but my file is too big for the free 32-bit version, and I've tried replacing fastq_maxee 1 with fastq_qmax 42, but that doesn't filter anything out. I'm a bit new to all this and I'm stuck. Any help would be greatly appreciated!

    -science panda

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