Picard InsertSizeMetrics Output Interpretation

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OlgaMikh

Junior Member

Join Date: Feb 2012

Posts: 9
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Picard InsertSizeMetrics Output Interpretation

04-25-2019, 06:40 AM

Hi,

I am looking at the data from my pilot Miseq Nano run performed on ATAC-seq libraries.
I wanted to see whether the fragment size distribution pattern corresponds to the one normally observed in ATAC libraries (nucleosome-free regions followed by nucleosomes), and ran Picard InsertSizeMetrics. I'm struggling with the interpretation of its output: what's he difference between FR count and RF count? Why am I getting distributions that are so different? Please see the plots attached. Thanks!
Attached Files

Insert size FR title.png (41.4 KB, 6 views)

Insert size RF title.png (35.4 KB, 5 views)
Tags: atac-seq, illumina, insertsize, picard

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