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Generally 4Gb per instance of sampe, 8Gb of Ram is not much in the sequencing world, consider running a pipeline were you run one lane at a time, I've posted one in my intro thread if you would like -
Bwa memory requirements for hg19
Hello everybody,
what is the minimum amount of memory people have to get successful alignments to the human genome with bwa? We're talking <15m Illumina transcriptome reads, 36-40bp.
The server is a Ubuntu 8.04 64bit with 8GB, and bwa has already caused it to slow down so much so that it needed to be rebooted.
Any thoughts would be welcome.
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Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
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