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It has been a long time and I don't remember exactly, but I think I solved the problem by trimming the polyG at the end of reads.
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Hi did you got any solution? I am stuck in the same position and trying to figure out
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Problem with DRAGEN Bio-IT Platform
I have a problem with DRAGEN Bio-IT Platform.
When I ran the map/alin and valiant caller pipeline, it stopped running by time out error.
I asked to illumina technical support, and changed the usage memory amount using this option.
" --bin_memory 60000000000 "
However, this option and more usage memory (200,000,000,000) did not solve the problem, and error was printed as below.
" The DRAGEN WatchDog service has detected a fault: Available free memory (1518 MB) is below minimum threshold (2048 MB) required by DRAGEN "
I have enough memory server and I did not run any other scripts at the same when I ran DRAGEN pipeline.
This probrem was happened when I input 193 Gbyte fastq.gz file.
Could you tell me how I can solve this problem and/or the cause of this problem?
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by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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