-
How about Newbler for the 454 reads? Not sure how it would deal w/ PacBio data, but if you use fasta + qual I think it could handle it (depending on read length). -
I guess it depends on how many reads you've got. For a moderate number of seuqences BLAT does a good job as well.
I used bwasw for 454 data as well and it worked out nicely.Leave a comment:
-
454: Extremely general question
All,
I have 454 and PacBio SRA data (that are cleaned of adaptors) that I'd like to align to a reference. I've tried bwasw, but when I look at the alignments in samtools tview, they are rather subpar as compared to data that has been reported by others on these sequences. Any suggestions for general approaches to aligning long reads? bwasw should work, correct? Better tools for dealing with these data?
-
Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
Long-read sequencing has...Today, 01:49 PM -
The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben MartÃnez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...11-06-2024, 07:24 PM
Leave a comment: