Hello! I've just started a PhD in DNA sequencing for the identification of cancer biomarkers and I was hoping to get some advice on bioinformatic methods. Part of my experimental design is to carry out Nanopore sequencing and I'm quite new to long-read sequencing and bioinformatics. I'd originally planned to use the 'easy' bioinformatic pipeline as advertised by Nanopore so I could practice/learn command line but not miss out on sequencing samples, however I've also noticed its not common to see it in journals or discussions.
Why is this? Would I be unable to publish data analysed with epi2me? Is it better if I sequence the samples THEN carry out bioinformatic analysis when I'm more confident in it?
Thanks!!
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Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
Long-read sequencing has seen remarkable advancements,...12-02-2024, 01:49 PM