I have an rna seq dataset and I am using Deseq2 to find differentially expressed genes between the two groups. However, I also want to remove genes in low counts by using a base mean threshold. I used pre-filtering to remove any genes that have no counts or only one count across the samples, however, I also want to remove those that have low counts compared to the rest of the genes. Is there a common threshold used for the basemean or a way to work out what this threshold should be?
Thank you
Thank you