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Thank you for your reply! If I want to do assembly afterwards, do I need to trim and filter the reads? Thank you very much! -
FastQC is a quality assessment program. It does not change any data or do actual analysis.
If you are planning to do alignments against a good reference then you don't strictly need to do any trimming/filtering. Aligners will soft clip any adapter sequence that may still be present.Leave a comment:
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What we can learn from FastQC reports
Hi All,
I have a question: for a relatively "good" FastQC report, do we still need to do data pre-processing?
For example, my FastQC report has good adapter content, which shows 0. Do we still need to trim the adapters or filter the reads? Thank you!
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